Ions5 tm xl
Web20 feb. 2024 · The DNA libraries was generated based on an IonS5TMXL (Thermo Fisher Scientific) (NegNE no. 1–5, SsNE no. 1–5, NegST no. 1–5, and SsST no.1-2) and the Illumina Novaseq 6000 platform (Illumina, USA) (SsST no. 3–5), and quantified via Qubit and Q-PCR. Single-end and paired-end raw reads were generated. WebThermo Fisher Scientific
Ions5 tm xl
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Web1 nov. 2024 · Based on IonS5™XL platform sequencing of respective samples targeting 16S rRNA gene that harbor V3-V4 conserved region revealed 1364 and 1254 microbial operational taxonomic units (OTUs) at ≥97% similarity and were classified into 23, 20 phyla; 70, 65 classes; 101, 87 orders; 189,180 families; 275, 271 genera and 94, 95 species. Web27 okt. 2024 · The purified amplicon were constructed for single-end sequencing based on the IonS5 TM XL sequencing platform. The reads were assigned to operational taxonomic units (OTUs) with a 97% similarity threshold and taxonomy assignment of the resulting OTU was carried out using the BLAST against the UNITE reference database.
WebHere, a high-throughput sequencing technique, IonS5TMXL was employed to analyze tobacco leaves infected by D. segeticola at different disease severity levels, ranging from … Web14 okt. 2024 · Here, a high-throughput sequencing technique, IonS5 TM XL was employed to analyze tobacco leaves infected by D. segeticola at different disease severity levels, ranging from T1G (least disease...
Web10 sep. 2024 · The sequencing platform was IonS5 TM XL. The single-end method was used to construct a small fragment library for single-terminal sequencing. 16s rRNA … WebContent from this work may be used under the terms of the CreativeCommonsAttribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
WebSingle-end sequencing (SE600) was performed on Thermo Fisher's IonS5TMXL sequencing platform to obtain high-throughput sequences. The total DNA extracted from the bacteria …
Web17 feb. 2024 · Cognitive impairment is associated with aging; however, the underlying mechanism remains unclear. Our previous study found that polyphenol-rich … darling harbour weather tomorrowWebThen, the extracted intestinal microbiota genomic was used as a template for PCR amplification with bacterial 16S rRNA gene universal primer 341F/806R. After … darling harbour weather 14 day forecastWeb10 mrt. 2024 · The sequencing library was constructed using the Ion Plus Fragment Library Kit 48 rxns (Thermo Scientific). After the constructed library was qualified by Qubit … bismarck grand theater showtimesWebAbstract: In order to analyze the effect of crop rotation on bacterial diversity and community structure in the rhizosphere soil of cotton,cotton was used as the research object,peanut … darling harbour weather forecastWeb13 mrt. 2024 · The rhizosphere bacterial communities were explored via amplicon sequencing of V3–V4 region of 16S rRNA region using IonS5™XL sequencing platform. The classified tags for 16S rRNA from both the samples were clustered into 1502 Microbial operational taxonomic units (OTUs) at 97% similarity with 1340 OTUs in Zea mays and … bismarck greenville ohioWeb7 apr. 2024 · The sequencing of samples was performed on an IonS5 TM XL sequencer by Novogene, Beijing, China. Processing of sequencing data was performed on extracted high-quality sequences using the Quantitative Insights … darling harbour to manly beachWeb8 jan. 2024 · In this study, 88 intestinal microbiota samples that were collected at 11-time points were sequenced using the IonS5 TM XL platform to determine the characteristic … bismarck grand theater